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Slot: org_count_qpcr_info

If qpcr was used for the cell count, the target gene name, the primer sequence and the cycling conditions should also be provided. (Example: 16S rrna; FWD:ACGTAGCTATGACGT REV:GTGCTAGTCGAGTAC; initial denaturation:90C_5min; denaturation:90C_2min; annealing:52C_30 sec; elongation:72C_30 sec; 90 C for 1 min; final elongation:72C_5min; 30 cycles)

URI: MIXS:0000099

Properties

Identifier and Mapping Information

Annotations

property value
Expected_value gene name;FWD:forward primer sequence;REV:reverse primer sequence;initial denaturation:degrees_minutes;denaturation:degrees_minutes;annealing:degrees_minutes;elongation:degrees_minutes;final elongation:degrees_minutes; total cycles

Schema Source

  • from schema: https://w3id.org/mixs-6-2-rc

LinkML Source

name: org_count_qpcr_info
annotations:
  Expected_value:
    tag: Expected_value
    value: gene name;FWD:forward primer sequence;REV:reverse primer sequence;initial
      denaturation:degrees_minutes;denaturation:degrees_minutes;annealing:degrees_minutes;elongation:degrees_minutes;final
      elongation:degrees_minutes; total cycles
  Preferred_unit:
    tag: Preferred_unit
    value: number of cells per gram (or ml or cm^2)
description: 'If qpcr was used for the cell count, the target gene name, the primer
  sequence and the cycling conditions should also be provided. (Example: 16S rrna;
  FWD:ACGTAGCTATGACGT REV:GTGCTAGTCGAGTAC; initial denaturation:90C_5min; denaturation:90C_2min;
  annealing:52C_30 sec; elongation:72C_30 sec; 90 C for 1 min; final elongation:72C_5min;
  30 cycles)'
title: organism count qPCR information
from_schema: https://w3id.org/mixs-6-2-rc
string_serialization: '{text};FWD:{dna};REV:{dna};initial denaturation:degrees_minutes;denaturation:degrees_minutes;annealing:degrees_minutes;elongation:degrees_minutes;final
  elongation:degrees_minutes; total cycles'
slot_uri: MIXS:0000099
multivalued: false
alias: org_count_qpcr_info
domain_of:
- HydrocarbonResourcesCores
- HydrocarbonResourcesFluidsSwabs
range: string
required: false
recommended: false