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Term: pcr conditions (pcr_cond)

Description of reaction conditions and components of polymerase chain reaction performed during library preparation.

URI: MIXS:0000049

Applicable Checklists and Extensions

NOTE: does not include Combinations (of checklists and extensions) that use pcr_cond.

Name Description Checklist/Extension
MimarksS Minimal Information about a Marker Sequence: survey Checklist
MimarksC Minimal Information about a Marker Sequence: specimen Checklist
Agriculture A collection of terms appropriate when sequencing samples obtained in an agri... Extension

Properties

  • Range: String
  • Cardinality: 0..1

Examples

Value
initial denaturation:94_3;annealing:50_1;elongation:72_1.5;final elongation:72_10;35
initial denaturation:94degC_1.5min

Annotations

  • Expected_value: initial denaturation:degrees_minutes;annealing:degrees_minutes;elongation:degrees_minutes;final elongation:degrees_minutes;total cycles

LinkML Source

name: pcr_cond
annotations:
  Expected_value:
    tag: Expected_value
    value: initial denaturation:degrees_minutes;annealing:degrees_minutes;elongation:degrees_minutes;final
      elongation:degrees_minutes;total cycles
description: Description of reaction conditions and components of polymerase chain
  reaction performed during library preparation.
title: pcr conditions
examples:
- value: initial denaturation:94_3;annealing:50_1;elongation:72_1.5;final elongation:72_10;35
- value: initial denaturation:94degC_1.5min
in_subset:
- sequencing
from_schema: https://w3id.org/mixs
keywords:
- condition
- pcr
string_serialization: initial denaturation:degrees_minutes;annealing:degrees_minutes;elongation:degrees_minutes;final
  elongation:degrees_minutes;total cycles
slot_uri: MIXS:0000049
alias: pcr_cond
domain_of:
- MimarksC
- MimarksS
- Agriculture
range: string