Term: pcr primers (pcr_primers)

PCR primers that were used to amplify the sequence of the targeted gene, locus or subfragment. This field should contain all the primers used for a single PCR reaction if multiple forward or reverse primers are present in a single PCR reaction. The primer sequence should be reported in uppercase letters

URI: MIXS:0000046

Applicable Checklists and Extensions

NOTE: does not include Combinations (of checklists and extensions) that use pcr_primers.

Name	Description	Checklist/Extension
MimarksS	Minimal Information about a Marker Sequence: survey	Checklist
Agriculture	A collection of terms appropriate when sequencing samples obtained in an agri...	Extension
MimarksC	Minimal Information about a Marker Sequence: specimen	Checklist

Properties

• Range: String

• Cardinality: 0..1

• Structured pattern: FWD:{ambiguous_nucleotides};REV:{ambiguous_nucleotides}

Examples

Value
FWD:GTGCCAGCMGCCGCGGTAA;REV:GGACTACHVGGGTWTCTAAT

LinkML Source

name: pcr_primers
description: PCR primers that were used to amplify the sequence of the targeted gene,
  locus or subfragment. This field should contain all the primers used for a single
  PCR reaction if multiple forward or reverse primers are present in a single PCR
  reaction. The primer sequence should be reported in uppercase letters
title: pcr primers
examples:
- value: FWD:GTGCCAGCMGCCGCGGTAA;REV:GGACTACHVGGGTWTCTAAT
in_subset:
- sequencing
from_schema: https://w3id.org/mixs
keywords:
- pcr
slot_uri: MIXS:0000046
alias: pcr_primers
domain_of:
- MimarksC
- MimarksS
- Agriculture
range: string
structured_pattern:
  syntax: FWD:{ambiguous_nucleotides};REV:{ambiguous_nucleotides}
  interpolated: true